Study on the Relation Patterns of Bitter leaf Juice Phytochemicals at Varying Temperature

Orlando Ketebu, Raphael Tari Samuel, Mayen Ben-Koko


Bitter leaf juice is known to have nutritional and therapeutic benefit which depends on its constituents for its efficiency when consumed.  In Nigeria, bitter leaf juice is prepared and consumed at room temperature and elevated temperatures for treatment of malaria, reducing blood sugar levels, as anti-oxidants, anti-bacterial and other numerous medicinal and pharmaceutical properties. The argument of what temperature is suitable for the juice consumption and its efficacy always arises. It becomes necessary to investigate how varying temperature affects the physiochemical and phytochemical properties of the juice, its bitter taste and the relationship patterns in which this variation takes place. This project work looks at the relationship patterns between the phytochemical properties of bitter leaf juice at varying temperature and their probably benefit. Laboratory quantitative analysis of some physical properties (pH, specific gravity and conductivity) and phytochemical components (alkaloids, tannins, saponnins, flavonoids, terpenoids and phenols) were carried out for bitter leaf juice samples heated within temperatures 40°C, 60°C, 80°C and 100°C and then compared to the quantitative analysis of the bitter leaf extract sample at room temperature (25°C). The result showed a significant reduction in the phytochemical constituents where alkaloids and phenols  reduces linearly with increasing temperature, tannins, saponnins, flavonoids and terpenoids  reduces with increasing temperature in polynomial form of second order. The pH and specific gravity also decreases as temperature increases in polynomial form of second order while the conductivity increases with increasing temperature. These change showed that the medicinal and pharmaceutical properties of the juice and bitter taste decreases with increasing heat. The bitter leaf juice becomes, more acidic, less dense than water and more conducting at high temperatures. Thus, the juice prepared at 25°C is best suited for nutritional and therapeutic benefit and if heat is to be applied to bitter leaf juice, the temperature 40°C is recommended since at this temperature, the composition of the phytochemical and physiochemical properties is not much different from that at 25°C.


Bitter leaf juice, temperature, physiochemical, phytochemical properties, relation pattern

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Bonsi, M.L.K., Osuji, P.O., Tuah, A.K.,Umunna,

M.N., Vernonia Amygdalina as supplement of teff straw

(Eragrostis tef) Fed to Ethiopian MenZ ship. Agroforestry Syst., 31:


Ogundare, A.O., 2011. Antibacterial properties `

Of the leaf extracts of Vernonia amygdalina, Ocimum

gratissimum, Corchorous olitorius and Manihot palmate. Journal of

Microbiology and Antimicrobials 3: 77 – 86.

Butler, G.W., Baily, R.W., 1973. Chemistry and

Biochemistry of herbage, Academic Press London, 1: Ix 639.

Ugwoke, C.E.C., Nzekwe, U., and Ameh, G.I.,

Phytochemical constituents and ethnobotany of the leaf

extract of bitter leaf (Vernonia amygdalina). Journal of

Pharmaceutical and Allied Sciences 7: 7-15.

Aliero, A.A., and Abdullahi, L. 2009. Effect of

Drying on the Nutrient Composition of Vernonia Amygdalina

Leaves. Journal of Phytology 1: 28–32.

Jim, M., and Stewart, T., 2017. Essentials of

Human Nutrition. Oxford; Oxford University Press. 130-185.

Atangwho I. J., Ebong P. E., Eyong E. U.,

Williams, I. O., Eteng M. U. and Egbung G. E., 2009.

Comparative chemical composition of leaves of some antidiabetic

medicinal plants: Azadirachta indica, Vernonia amygdalina and

Gongronema latifolium. African Journal of Biotechnology., 8: 4685

– 4689.

Atangwho I.J., Ebong P.E., Eyong E.U and

Egbung, G.E., 2010. Combined Extracts of Vernonia amygdalina

and Azadirachta indica may substitute insulin requirement in the

management of type 1 diabetes. Research Journal of Medical

Science. 5: 35-39.

Yeap, S.K., Ho W.H., Beh B.K., Liang W.S.,

Huynh, K., Abdul N.Y.H and Alitheen N.B. (2010) Vernonia

amygdalina, an Ethnoveterinary and Ethnomedical used green

vegetable with multiple bioactivities’. Journal of Medicinal Plants

Research 4 (25); 2787 – 2812.

Agbogidi, O.M., and Akpomorine, M.O., 2013.

Health and Nutritional Benefits of Bitter leaf (Vernonia Amygdalina

Del.) International Journal of Applied Pharmaceutical Sciences and

Biological Sciences. 2: 164 – 170.

Bergman, K. N., 2000. Regulation of

Cholesterol biosynthesis. Journal of Cholesterolemia., 3: 220-224.

Offor, C.E., 2014. Comparative Chemical

Analyses of Vernonia amygdalina and Azadirachta indica Leaves.

Journal of Pharmacy and Biological Sciences 9: 2278 – 3008.

Udochukwu, U., Omeje, F.I., Uloma I.S., and

Oseiwe F. D., 2015. Phytochemical Analysis of Vernonia

Amygdalina and Ocimum Gratissimum Extracts and Their

Antibacterial Activity on Some Drug Resistant Bacteria, American

Journal of Research Communication 3: 225-235.

Ejoh, A.R., Tanya, A.N., Djuikwo, N.V. and

Mbofung, C.M., 2005. Effect of processing and preservation

methods on Vitamin C and total carotenoid levels of some Vernonia

(bitter leaf) species. African Journal of Food, and nutritional Science,

: 2-11

Harborne, J.B., 1973. Phytochemical methods,

London. Chapman and Hall, Ltd. 49-188.

Pearson, D., 1976. Chemical analysis of foods.

th Edition, Henry Edward company, Churchchill, Livingstone,

London, 218-336.

Boham, BA., Kocipai-Abyazan, R., 1974.

Flavonoids and condensed tannins from leaves of Hawaiian

vaccinium vaticulatum and V. calycinium. Pacific Science. 48: 458-

Obdoni, B.O., Ochuk. P.O., 2001.

Phytochemical studies and comparative efficacy of the crude

extracts of some Homostatic plants in Edo and Delta States of

Nigeria. Global J. Pure Appl. Sci. 8: 203-208.

Ferguson, N. M., 1956. A Text book of

Pharmacognosy. Mac Milan Company, New Delhi. 191.

Abosi, A.O., Raseroka, B.H., 2003. In vivo-

antimalarial activity of Vernonia amygdalina. Br. J. of Biomed. Sci.,

: 89-91.

Mensah, J.K., Okoli, R.I., Ohaju-Obodo, J.O.

And Eifediyi. K., 2008. Phytochemical, nutritional and medical

properties of some leafy vegetables consumed by Edo people of

Nigeria. African Journal of Biotechnology. 7: 2304 – 2309.

Ebenezer, O.F., and Olatunde, O., 2011

Antioxidant and Chemo-preventive Properties of Vernonia

amygdalina and Garcinia bi-flavonoid. International. Journal of

Environmental Resources and Public Health, 8: 2533 – 2555.

Saalu, L.C., Akunna, G.G. and Ajayi, J.O.,

Modulating Role of Bitter Leaf on Spermatogenic and

Steroidogenesis Functions of the Rat Testis. American Journal of

Biochemistry and Molecular Biology, 3: 314-321.

Chung, K.T., Wong, T.Y., Haung, Y.W., and

Lin, Y., 1998. Tannin and Human Health: A review, Food Science

and Nutrition, 38: 421-464

Eleazu C.O., Eleazu, K.C., Awa. E. and

Chukwuma, S.C., 2012. Comparative study of the phytochemical

composition of the leaves of five Nigerian medicinal plants, Journal

of Biotechnology and Pharmaceutical Research. 3: 42 – 46

Francois, L., Jeanne, P.U., Claude, B., and

Emmanuel B.S., 2017. Antimicrobial Activity

And Phytochemicals Analysis of Vernonia

aemulans, Vernonia amygdalina, Lantana

camara and Markhamia lutea Leaves as

Natural Beer Preservatives. American

Journal of Food Technology, 12: 35-42.

Rao, A.V. and Sung, M.K.., 1995. Saponin as

Anticarcinogens. Journal of Nutrition, 125: 717-724

Iwalewa, E.O., Adewunmi C.O., Omisore,

N.O., Adebanji, O.A., Azike, C.K., Adigun, A.O., Adesina, O.A.

and Olowoyo O.G.J., 2005. Pro and antioxidant effects and

cytoprotective potentials of nine edible vegetables in southwest

Nigeria, Journal of Medical Food 8: 539 – 544.



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